Bachelor projects in Chemistry
I. Synthesis of hydrophobic building blocks for automated DNA and RNA synthesis:
The project will focus on chemical synthesis of hydrophobic lipid building blocks which are used as lipid building blocks in automated synthesis of lipidated DNA or RNA oligonuclotides (LiNAs). The lipidated DNAs or RNAs will be used in many other projects as bioactive LiNAs in medicinal chemistry projects or as supramolecular „zippers“ in programmed chemistry.
References:
1.) O. Ries, P. M. G. Löffler, S. Vogel, Convenient synthesis and application of versatile nucleic acid lipid membrane anchors in the assembly and fusion of liposomes, Org. Biomol. Chem., 2015, 9673-9680.
2.) O. Ries, P. M. G. Löffler, A. Rabe, J. J. Malavan and S. Vogel, Efficient liposome fusion mediated by lipid–nucleic acid conjugates. Org. Biomol. Chem. 2017, 15, 8936–8945.
II. Synthesis of hydrophobic building blocks for automated peptide synthesis:
The project will focus on chemical synthesis of hydrophobic peptide building blocks which are used as lipid building blocks in microwave assisted automated synthesis of lipidated peptides (LiPs). The lipidated peptides will be used in other projects as bioactive LiPs in medicinal chemistry projects as selective ligands targeting receptors or cell-penetrating peptides (CPPs) in lipid based formulations.
References:
1.) S. Cogoi, U. Jakobsen, E. B. Pedersen, S. Vogel & L. E. Xodo, Lipid-modified G4-decoy oligonucleotide anchored to nanoparticles: delivery and bioactivity in pancreatic cancer cells Sci. Rep. 2016, 6, 1-13.
2.) A. Ferino, G. Miglietta, R. Picco, S. Vogel, J. Wengel and L. E. Xodo, MicroRNA Therapeutics: Design of single-stranded miR-216b mimics to target KRAS in pancreatic cancer cells. RNA Biology, 2018 15:10, 1273-1285.
III. Microwave assisted automated synthesis of (bioactive) peptides
The project will focus on automated microwave assisted automated synthesis of lipidated peptides (LiPs). The lipidated peptides will synthesized and purified by HPLC and characterized by circular dichroism (CD) or surface plasmon resonance (SPR) be used in other projects as bioactive LiPs in medicinal chemistry projects as selective ligands targeting receptors or cell-penetrating peptides (CPPs) in lipid based formulations.
References:
1.) S. Cogoi, U. Jakobsen, E. B. Pedersen, S. Vogel & L. E. Xodo, Lipid-modified G4-decoy oligonucleotide anchored to nanoparticles: delivery and bioactivity in pancreatic cancer cells Sci. Rep. 2016, 6, 1-13.
2.) A. Ferino, G. Miglietta, R. Picco, S. Vogel, J. Wengel and L. E. Xodo, MicroRNA Therapeutics: Design of single-stranded miR-216b mimics to target KRAS in pancreatic cancer cells. RNA Biology, 2018 15:10, 1273-1285.